A One Pot, One Step, Precision Cloning Method with High Throughput Capability
نویسندگان
چکیده
منابع مشابه
A One Pot, One Step, Precision Cloning Method with High Throughput Capability
Current cloning technologies based on site-specific recombination are efficient, simple to use, and flexible, but have the drawback of leaving recombination site sequences in the final construct, adding an extra 8 to 13 amino acids to the expressed protein. We have devised a simple and rapid subcloning strategy to transfer any DNA fragment of interest from an entry clone into an expression vect...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2008
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0003647